[ Title ]
- Recent progress in developing proximity ligation assays for pathogen
detection
[ Journal ]
- EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
[ Abstract ]
- The effective management of infectious diseases depends on the early
detection of the microbes responsible, since pathogens are most
effectively eliminated in the initial stages of infection. Current
immunodiagnostic methods lack the sensitivity for earliest possible
diagnosis. Nucleic acid-based tests (NATs) are more sensitive, but the
detection of microbial DNA does not definitively prove the presence of a
viable microorganism capable of causing a given infection. Proximity
assays combine the specificity of antibody-based detection of proteins
with the sensitivity and dynamic range of NATs, and their use may allow
earlier as well as more clinically relevant detection than is possible
with current NATs or immunoassays. However, the full potential of
proximity assays for pathogen detection remains to be fulfilled, mainly
due to the challenges associated with identifying suitable antibodies
and antibody combinations, sensitivity issues arising from non-specific
interactions of proximity probes and the longer incubation times
required to carry out the assays.
[ URL ]
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